There simply is NO difference between Hemoglobin and Hematocrit by means of clinical information! It is actually not measured directly! The analyser multiplies the red blood cell count by their mean corpuscular volume. Hematocrit levels in contrast are actually calculated by an automated analyzer. The concentration of haemoglobin is then measured by spectrophotometry using the methemoglobin cyanide method. Within the machine, the red blood cells are broken down to get the haemoglobin into a solution. Hemoglobin levels are mostly measured by automated machines designed to perform different tests in blood. So here's the question: What actually is the difference between Hb and Hct? Do we need to measure both in clinical practice? 'Hct is better to determine dilution of the patient' or 'Acute blood loss is better determined by Hb than Hct'. but then there is always someone making a great deal of differentiating between the two parameters and making all sort of diagnostic conclusions. Some clinicians look at haemoglobin levels, others prefer hematocrit levels. These two haematological lab-parameters are part of our daily life at work and are mostly measured together. It starts in medical school, regularly appears in your medical training, sneaks around nursing schools and is an impetus for discussions in the ICU: The great myths about Hemoglobin (Hb) and Hematocrit (Hct).
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